We are interested in the organization and assembly of the nuclear envelope during development and cell proliferation. The nuclear envelope forms a highly structured barrier that separates the nuclear interior from the rest of the cell. It is composed of a concentric double membrane that is penetrated by nuclear pores, which serve as channels for transport between the nucleus and the cytoplasm.


The reformation of the nucleus during mitosis is one of the most dramatic structural and functional changes in higher eukaryotic cells. Upon entering mitosis the complex architecture of the nuclear envelope completely breaks down to allow chromosomes to be captured by the mitotic spindle which then accurately partitions them to daughter cells. Once segregation is accomplished, the interphase architecture is re-established to enable perpetuation of genomic information.


Our lab is focusing on understanding the molecular basis of nuclear assembly and its regulation during cell division. The formation of the nuclear envelope is a step-wise process of remarkable complexity involving chromatin decondensation, membrane reorganization, and nuclear pore complex assembly. We are using live cell imaging, biochemistry and genetic tools, as well as Xenopus leavis and mammalian cell-free systems, to study various aspects of nuclear formation.


Additionally we are interested in the complexity of nuclear envelope function during muscle- and neuronal cell differentiation. Mammalian nuclei are complex organelles, whose functions depend largely on a spatial, higher order organization of chromatin. Anchorage of chromatin at the nuclear periphery and its three-dimensional organization within the nuclear interior may regulate cell type- and differentiation-specific gene expression. The recent identification of mutations in nuclear envelope proteins in a number of human diseases highlights the importance of understanding nuclear envelope assembly and structure.